Array biosensor for simultaneous detection of multiple analytes

Frances S. Ligler; Joel P. Golden; Chris A. Rowe-Taitt; James P. Dodson

doi:10.1117/12.426740

10 May 2001 Array biosensor for simultaneous detection of multiple analytes

Frances S. Ligler, Joel P. Golden, Chris A. Rowe-Taitt, James P. Dodson

Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426740
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

The array biosensor has been developed for simultaneous analysis of multiple samples for multiple analytes. A patterned array of capture antibodies is immobilized on the surface of a planar waveguide and a sandwich immunoassay conducted using a cocktail of fluorescent tracer antibodies. Upon excitation of the fluorescent label using a 635 nm diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. The assays are fast, sensitive, and specific.

Citation Download Citation

Frances S. Ligler, Joel P. Golden, Chris A. Rowe-Taitt, and James P. Dodson "Array biosensor for simultaneous detection of multiple analytes", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); https://doi.org/10.1117/12.426740

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