Pulsewidth-dependent nature of laser-induced DNA damage in RPE cells

Rebecca M. Hall; Randolph D. Glickman; Benjamin A. Rockwell; Neeru Kumar; Gary D. Noojin

doi:10.1117/12.434700

9 July 2001 Pulsewidth-dependent nature of laser-induced DNA damage in RPE cells

Rebecca M. Hall, Randolph D. Glickman, Benjamin A. Rockwell, Neeru Kumar, Gary D. Noojin

Proceedings Volume 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical; (2001) https://doi.org/10.1117/12.434700
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Ultrashort pulse laser radiation may produce cellular damage through unique mechanisms. Primary cultures of bovine retinal pigment epithelial (RPE) cells were exposed to the out put of a Ti:Sapphire laser producing 30 fs (mode-locked) pulses, 44 amplified fs pulses, or continuous wave exposures at 800 nm. Laser exposures at and below the damage threshold were studied. DNA damage was detected using single cell gel electrophoresis (comet assay). Unexposed (control) cells produced short tails with low tail moments. In contrast, all laser-exposed cells showed some degree of DNA fragmentation, but the size and shape of the resulting comets differed among the various modalities. CW-exposed cells produced generally light and relatively compact tails, suggesting fewer and larger DNA fragments, while mode-locked laser exposures (30 fs pulses) resulted in large and diffuse comets, indicating the DNA was fragmented into many very small pieces. Work is continuing to define the relationship of laser pulsewidth and intensity with the degree of DNA fragmentation. These results suggest that DNA damage may result from multiple mechanisms of laser-cell interaction, including multiphoton absorption.

Citation Download Citation

Rebecca M. Hall, Randolph D. Glickman, Benjamin A. Rockwell, Neeru Kumar, and Gary D. Noojin "Pulsewidth-dependent nature of laser-induced DNA damage in RPE cells", Proc. SPIE 4257, Laser-Tissue Interaction XII: Photochemical, Photothermal, and Photomechanical, (9 July 2001); https://doi.org/10.1117/12.434700

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