Optoelectronic closed-loop auto-oscillator for fluorescence lifetime detection: a new fluorimetry technique with applications to chemical/biosensors

Michael J. O'Brien II; Emmanuil M. Rabinovich; Tengiz Svimonishvili; Steven R. J. Brueck; Gabriel P. Lopez

doi:10.1117/12.429336

13 June 2001 Optoelectronic closed-loop auto-oscillator for fluorescence lifetime detection: a new fluorimetry technique with applications to chemical/biosensors

Michael J. O'Brien II, Emmanuil M. Rabinovich, Tengiz Svimonishvili, Steven R. J. Brueck, Gabriel P. Lopez

Author Affiliations +

Proceedings Volume 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring; (2001) https://doi.org/10.1117/12.429336
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

We present a new approach to excited state fluorescence lifetime-based measurements which is inexpensive and highly sensitive. The detection system consists of a closed-loop optoelectronic arrangement containing an intermediate frequency resonance amplifier, a fluorescence excitation light source (for example, a light emitting diode or a semiconductor laser), a fiber optic delay line, and a photodetector. The system exhibits self-oscillations in the vicinity of the frequency (Omega) approximately 1/(tau) (where (tau) is the excited state lifetime) which manifest themselves as modulation of the light. Changes in the excited state lifetime alter the phase delay of the loop, which in turn causes a frequency shift in the modulation signal. The frequency shift can be measured very precisely with a frequency counter. With appropriate averaging, this technique can yield sub-picosecond resolution of shifts in lifetime. This technique is suited for chemical/biological sensing applications, and can be easily duplicated for chemical/biological sensor arrays.

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Michael J. O'Brien II, Emmanuil M. Rabinovich, Tengiz Svimonishvili, Steven R. J. Brueck, and Gabriel P. Lopez "Optoelectronic closed-loop auto-oscillator for fluorescence lifetime detection: a new fluorimetry technique with applications to chemical/biosensors", Proc. SPIE 4263, Optical Diagnostics and Sensing of Biological Fluids and Glucose and Cholesterol Monitoring, (13 June 2001); https://doi.org/10.1117/12.429336

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