Comparative intrinsic and enhanced total photoluminescence of endospore material

John E. Anderson; Stanley Thomas Webb; Robert L. Fischer; Karen Kester; Clint Smith

doi:10.1117/12.456963

22 February 2002 Comparative intrinsic and enhanced total photoluminescence of endospore material

John E. Anderson, Stanley Thomas Webb, Robert L. Fischer, Karen Kester, Clint Smith

Proceedings Volume 4576, Advanced Environmental Sensing Technology II; (2002) https://doi.org/10.1117/12.456963
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States

Abstract

Two techniques are compared using total luminescence spectroscopy to detect endospore material in preparations equivalent to 3.0 x 10⁵/ml spores. The first method applied intrinsic, steady-state photoluminescence for detection. The second approach using a binding fluorochrome derived from 4-p-dimethylaminostyrylpyridinium (DASP) to signal the presence of spore material. Comparative fluorescence emission signatures (excited at 469 nm) showed greater calibrated signal recovery (4x10⁶ cps) for spore material at longer wavelengths using DASP. The intrinsic fluorescence emission of endospores (excited at 346 nm) occurred at shorter wavelengths and showed a reduced calibrated intensity (1.4 x 10⁵ counts per second (cps). One major advantage of DASP appears to be its longer wavelength excitation (469 nm) that is out of the range of associated biological materials that compete for absorption at shorter UV wavelengths.

Citation Download Citation

John E. Anderson, Stanley Thomas Webb, Robert L. Fischer, Karen Kester, and Clint Smith "Comparative intrinsic and enhanced total photoluminescence of endospore material", Proc. SPIE 4576, Advanced Environmental Sensing Technology II, (22 February 2002); https://doi.org/10.1117/12.456963

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