Polarization-based fluorescent method for enhanced analytical determination of mixed fluorophores in fluid

Yuan Yan; Michael L. Myrick

doi:10.1117/12.456957

22 February 2002 Polarization-based fluorescent method for enhanced analytical determination of mixed fluorophores in fluid

Yuan Yan, Michael L. Myrick

Author Affiliations +

Proceedings Volume 4576, Advanced Environmental Sensing Technology II; (2002) https://doi.org/10.1117/12.456957
Event: Environmental and Industrial Sensing, 2001, Boston, MA, United States

Abstract

Real-time mixture analysis is widely needed in environmental monitoring, kinetics analysis, and industrial process monitoring etc. Conventional methods for mixture analysis such as HPLC, HPLC/MS, or GC/MS, are not good for real-time purposes. Recently, we have demonstrated fluorescence polarization could be used as an additional dimension with which to distinguish fluorescent molecules by the introduction of some specific micelles. In this report, a novel method, the polarized fluorescence spectrometry including polarization information, has been proposed and used for simultaneous determination of fluorescein (FLR) and rhodamine 123 hydrate (R123H) without spectral dispersion. This could also be used for enhanced analytical determination of mixed fluorophores with 2-D fluorescence (excitation-emission matrix). At this time, conventional 2-D fluorescence spectra already provide enough information for principal component regression (PCR) or parallel factor analysis (PARAFAC) to distinguish four fluorophores: FLR, R123H, rhodamine 6G (R6G), and rhodamine B (RB), even FLR and R123H have a very similar 2-D fluorescence spectra.

Citation Download Citation

Yuan Yan and Michael L. Myrick "Polarization-based fluorescent method for enhanced analytical determination of mixed fluorophores in fluid", Proc. SPIE 4576, Advanced Environmental Sensing Technology II, (22 February 2002); https://doi.org/10.1117/12.456957

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