Peptide nucleic acid (PNA) biosensors for DNA recognition

Jiong Li; Hong Wang; Lu Cheng; Yujie Zhao; Heping Liu; Nongyao He; Zuhong Lu

doi:10.1117/12.444753

15 October 2001 Peptide nucleic acid (PNA) biosensors for DNA recognition

Jiong Li, Hong Wang, Lu Cheng, Yujie Zhao, Heping Liu, Nongyao He, Zuhong Lu

Proceedings Volume 4601, Micromachining and Microfabrication Process Technology and Devices; (2001) https://doi.org/10.1117/12.444753
Event: International Symposium on Optoelectonics and Microelectronics, 2001, Nanjing, China

Abstract

PNA has demonstrated remarkable hybridization properties towards complementary oligonucleotides. In this paper, we tested the PNA's ability of selectively discriminating against single-base mismatches. Efforts were paid to optimizing the fabrication of PNA biosensors and its hybridization to achieve better discriminating capacity. Different factors were studied such as immobilization concentration, target concentration, ion strength and hybridization temperature. An optimized procedure was produced to reliably detect DNAs with single-base mismatches in the sequences. We synthesized two 16 mer PNAs with single-base mismatch to hybridizing with complementary DNA. The complementary PNA showed 8.6 fold stronger signal intensity than mismatch one under our optimized procedure.

Citation Download Citation

Jiong Li, Hong Wang, Lu Cheng, Yujie Zhao, Heping Liu, Nongyao He, and Zuhong Lu "Peptide nucleic acid (PNA) biosensors for DNA recognition", Proc. SPIE 4601, Micromachining and Microfabrication Process Technology and Devices, (15 October 2001); https://doi.org/10.1117/12.444753

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available