Multiwavelength TCSPC lifetime imaging

Wolfgang Becker; Axel Bergmann; Christoph Biskup; Thomas Zimmer; Nikolaj Kloecker; Klaus Benndorf

doi:10.1117/12.470679

17 June 2002 Multiwavelength TCSPC lifetime imaging

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, Klaus Benndorf

Proceedings Volume 4620, Multiphoton Microscopy in the Biomedical Sciences II; (2002) https://doi.org/10.1117/12.470679
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

We present a novel time-correlated single photon counting (TCPSC) imaging technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over time, the x-y coordinates of the scanning area and the detector channel number. The histogramming process avoids any time gating or wavelength scanning and therefore yields a near-perfect counting efficiency. Applied to resonance energy transfer (RET) measurements, the setup is capable to record time-resolved fluorescence decays for the donor and the acceptor simultaneously.

Citation Download Citation

Wolfgang Becker, Axel Bergmann, Christoph Biskup, Thomas Zimmer, Nikolaj Kloecker, and Klaus Benndorf "Multiwavelength TCSPC lifetime imaging", Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); https://doi.org/10.1117/12.470679

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