Fluorescence imaging of angiogenesis in green fluorescent protein-expressing tumors

Meng Yang; Eugene Baranov; Ping Jiang; Xiao-Ming Li; Jin Wei Wang; Lingna Li; Shigeo Yagi; A. R. Moossa; Robert M. Hoffman

doi:10.1117/12.468331

28 May 2002 Fluorescence imaging of angiogenesis in green fluorescent protein-expressing tumors

Meng Yang, Eugene Baranov, Ping Jiang, Xiao-Ming Li, Jin Wei Wang, Lingna Li, Shigeo Yagi, A. R. Moossa, Robert M. Hoffman

Author Affiliations +

Proceedings Volume 4622, Optical Diagnostics of Living Cells V; (2002) https://doi.org/10.1117/12.468331
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

The development of therapeutics for the control of tumor angiogenesis requires a simple, reliable in vivo assay for tumor-induced vascularization. For this purpose, we have adapted the orthotopic implantation model of angiogenesis by using human and rodent tumors genetically tagged with Aequorea victoria green fluorescent protein (GFP) for grafting into nude mice. Genetically-fluorescent tumors can be readily imaged in vivo. The non-luminous induced capillaries are clearly visible against the bright tumor fluorescence examined either intravitally or by whole-body luminance in real time. Fluorescence shadowing replaces the laborious histological techniques for determining blood vessel density. High-level GFP-expressing tumor cell lines made it possible to acquire the high-resolution real-time fluorescent optical images of angiogenesis in both primary tumors and their metastatic lesions in various human and rodent tumor models by means of a light-based imaging system. Intravital images of angiogenesis onset and development were acquired and quantified from a GFP- expressing orthotopically-growing human prostate tumor over a 19-day period. Whole-body optical imaging visualized vessel density increasing linearly over a 20-week period in orthotopically-growing, GFP-expressing human breast tumor MDA-MB-435. Vessels in an orthotopically-growing GFP- expressing Lewis lung carcinoma tumor were visualized through the chest wall via a reversible skin flap. These clinically-relevant angiogenesis mouse models can be used for real-time in vivo evaluation of agents inhibiting or promoting tumor angiogenesis in physiological micro- environments.

Citation Download Citation

Meng Yang, Eugene Baranov, Ping Jiang, Xiao-Ming Li, Jin Wei Wang, Lingna Li, Shigeo Yagi, A. R. Moossa, and Robert M. Hoffman "Fluorescence imaging of angiogenesis in green fluorescent protein-expressing tumors", Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); https://doi.org/10.1117/12.468331

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KEYWORDS

Tumors

Green fluorescent protein

Luminescence

Blood vessels

Tumor growth modeling

In vivo imaging

Lung

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