Use of in vitro OmniPlex libraries for high-throughput comparative genomics and molecular haplotyping

Emmanuel Kamberov; Irina Sleptsova; Stephen Suchyta; Eric D. Bruening; William Ziehler; Julie Seward Nagel; John P. Langmore; Vladimir Makarov

doi:10.1117/12.472062

21 June 2002 Use of in vitro OmniPlex libraries for high-throughput comparative genomics and molecular haplotyping

Emmanuel Kamberov, Irina Sleptsova, Stephen Suchyta, Eric D. Bruening, William Ziehler, Julie Seward Nagel, John P. Langmore, Vladimir Makarov

Author Affiliations +

Proceedings Volume 4626, Biomedical Nanotechnology Architectures and Applications; (2002) https://doi.org/10.1117/12.472062
Event: International Symposium on Biomedical Optics, 2002, San Jose, CA, United States

Abstract

OmniPlex Technology is a new approach to genome amplification and targeted analysis. Initially the entire genome is reformatted into small, amplifiable molecules called Plexisomes, which represent the entire genome as an OmniPlex Library. The whole genome can be amplified en masse using universal primers; using locus-specific primers, regions as large as 50 kb can be amplified. Amplified Plexisomes can be analyzed using conventional methods such as capillary sequencing and microarray hybridization. The advantages to using OmniPlex as the 'front-end' for conventional analytical instruments are that a) the initial copy number of the analytes can be increased to achieve better signal-to-noire ratio, b) only a single priming site is used and c) up to 20 times fewer biochemical reactions and oligonucleotides are necessary to amplify a large region, compared to conventional PCR. These factors make OmniPlex more flexible, faster, and less expensive than conventional technologies. OmniPlex has been applied to targeted sequencing of human, animal, plant, and microorganism genomes. In addition, OmniPlex is inherently able to haplotype large regions of human DNA to accelerate target discovery and pharmacogenomics. OmniPlex will be a key tool for delivery of improved crops and livestock, new pharmaceutical products, and personalized medicine.

Citation Download Citation

Emmanuel Kamberov, Irina Sleptsova, Stephen Suchyta, Eric D. Bruening, William Ziehler, Julie Seward Nagel, John P. Langmore, and Vladimir Makarov "Use of in vitro OmniPlex libraries for high-throughput comparative genomics and molecular haplotyping", Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); https://doi.org/10.1117/12.472062

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available