Mechanism of photobiological effects of psoralens: the involvement of photooxidized psoralens as reactive intermediate species in induction of photosensitized hemolysis and skin erythema

Eugene P. Lysenko; Alla A. Kyagova; Alexander Ya. Potapenko

doi:10.1117/12.475620

16 July 2002 Mechanism of photobiological effects of psoralens: the involvement of photo-oxidized psoralens as reactive intermediate species in induction of photosensitized hemolysis and skin erythema

Eugene P. Lysenko, Alla A. Kyagova, Alexander Ya. Potapenko

Author Affiliations +

Proceedings Volume 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III; (2002) https://doi.org/10.1117/12.475620
Event: Saratov Fall Meeting 2001, 2001, Saratov, Russian Federation

Abstract

Psoralens combined with UVA irradiation are used for the treatment of skin and autoimmune diseases. In the present paper the involvement of psoralen photooxidation products (POP) into induction of PUVA-erythema was studied. Under low fluence rate (LFR) UVA-irradiation and/or at low psoralen concentrations POP-products were predominantly formed, which were detected by spin trp method and possessed immunosuppressive activity. Under high fluence rate (HFR) UVA-irradiation and/or at high psoralen concentrations POP₂-products were predominantly formed, which could be detected by Fe(II)-induced chemiluminescence and possessed hemolytic activity. Both PUVA- and POP-induced hemolysis of erythrocytes as well as PUVA-erythema were strongly activated with the increase in UVA-fluence rate. Both LFR and HFR PUVA-hemolysis as well as POP-hemolysis were strongly activated by Fe(II)-ions, bivalent cation chelators produced different effects on these processes depending on UVA fluence rate. Ethylenediaminetetraacetate (EDTA), and o- fenanthroline were found to inhibit LFT PUVA-erythema and enhanced HFR PUVA-erythema. Similar regulatory effects of EDTA were found for PUVA- and POP-induced hemolysis. EDTA inhibited LFT PUVA-hemolysis and activated HFR PUVA- and POP-hemolysis that suggests a participation of POP₁- products in induction of LFR PUVA-hemolysis and LFT PUVA- erythema and POP₂-products in induction of HFR PUVA- hemolysis and HFR PUVA-erythema.

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Eugene P. Lysenko, Alla A. Kyagova, and Alexander Ya. Potapenko "Mechanism of photobiological effects of psoralens: the involvement of photo-oxidized psoralens as reactive intermediate species in induction of photosensitized hemolysis and skin erythema", Proc. SPIE 4707, Saratov Fall Meeting 2001: Optical Technologies in Biophysics and Medicine III, (16 July 2002); https://doi.org/10.1117/12.475620

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