Multiphoton imaging using a Ti:sapphire regenerative amplifier

Patrick Theer; Mazahir T. Hasan; Winfried T. Denk

doi:10.1117/12.499783

9 October 2003 Multiphoton imaging using a Ti:sapphire regenerative amplifier

Patrick Theer, Mazahir T. Hasan, Winfried T. Denk

Proceedings Volume 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging; (2003) https://doi.org/10.1117/12.499783
Event: European Conferences on Biomedical Optics 2003, 2003, Munich, Germany

Abstract

It is shown that two-photon fluorescence images can be obtained almost throughout the entire grey-matter layers of the mouse neocortex by using optically amplified femtosecond pulses. The maximum imaging depth is not limited by the available excitation power but instead by the generation of out-of focus fluorescence.

Citation Download Citation

Patrick Theer, Mazahir T. Hasan, and Winfried T. Denk "Multiphoton imaging using a Ti:sapphire regenerative amplifier", Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); https://doi.org/10.1117/12.499783

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