Optical control of a rhodopsin-based switch

Ben Ovryn; Xiang Li; Hillel Chiel; Stefan Herlitze

doi:10.1117/12.578942

13 July 2004 Optical control of a rhodopsin-based switch

Ben Ovryn, Xiang Li, Hillel Chiel, Stefan Herlitze

Proceedings Volume 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI; (2004) https://doi.org/10.1117/12.578942
Event: Biomedical Optics 2004, 2004, San Jose, CA, United States

Abstract

A preliminary result supports the feasibility of using visible light to modulate the membrane potential of a cell. Human embryonic kidney cells (HEK293) were transfected with vertebrate rhodopsin and a gradient inward rectifying potassium (GIRK) channel. Whole cell patch clamp recordings of HEK293 cells exposed to 9-cis retinal showed that illumination increases the potassium current compared with recordings obtained in the dark. When combined with a rapid scanning device, this approach has the potential to control the activity of many neurons.

Citation Download Citation

Ben Ovryn, Xiang Li, Hillel Chiel, and Stefan Herlitze "Optical control of a rhodopsin-based switch", Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); https://doi.org/10.1117/12.578942

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