Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content

Svetlana N. Klueva; Vladimir N. Korsukov; Tatyana N. Schukovskaya; Alexander L. Kravtsov

doi:10.1117/12.579011

20 August 2004 Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content

Svetlana N. Klueva, Vladimir N. Korsukov, Tatyana N. Schukovskaya, Alexander L. Kravtsov

Author Affiliations +

Proceedings Volume 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V; (2004) https://doi.org/10.1117/12.579011
Event: Saratov Fall Meeting 2003, 2003, Saratov, Russian Federation

Abstract

Using flow cytometry (FCM) the influence of exogenous serotonin on culture growth, DNA content and fluorescence intensity of cells binding FITC-labelled plague polyclonal immunoglobulins was studied in Yersinia pestis EV (pFra⁺, pCad⁺, pPst⁺), Yersinia pestis KM218 (pFra^-, pCad^-, pPst^-), Yersinia pestis KM 216 (pFra^-, pCad^-, pPst⁺). The results have been obtained by FCM showed serotonin accelerated Yersinia pestis EV (pFra⁺, pCad⁺, pPst⁺), Yersinia pestis KM218 (pFra^-, pCad^-, pPst^-) culture growth during cultivation in Hottinger broth pH 7.2 at 28°C at concentration of 10^-5 M. The presence of 10^-5 M serotonin in nutrient broth could modulate DNA content in 37°C growing population of plague microbe independently of their plasmid content. Serotonin have been an impact on the distribution pattern of the cells according to their phenotypical characteristics, which was reflected in the levels of population heterogeneity in the intensity of specific immunofluorescence determined by FMC.

Citation Download Citation

Svetlana N. Klueva, Vladimir N. Korsukov, Tatyana N. Schukovskaya, and Alexander L. Kravtsov "Effect of serotonin on the expression of antigens and DNA levels in Yersinia pestis cells with different plasmid content", Proc. SPIE 5474, Saratov Fall Meeting 2003: Optical Technologies in Biophysics and Medicine V, (20 August 2004); https://doi.org/10.1117/12.579011

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