Optical Microscopic Tomography

Satoshi Kawata; Yusuke Touki; Shigeo Minami

doi:10.1117/12.949566

25 October 1985 Optical Microscopic Tomography

Satoshi Kawata, Yusuke Touki, Shigeo Minami

Proceedings Volume 0558, Inverse Optics II; (1985) https://doi.org/10.1117/12.949566
Event: 29th Annual Technical Symposium, 1985, San Diego, United States

Abstract

A new principle to obtain sectional images of a thick specimen by a conventional optical microscope without physically slicing is developed. The optical microscope with an off-axis pupil is used to project a 3-D distribution of the specimen in various directions within the numerical aperture of the objective lens. Since the system is the strictly angularly-limited one, a strong constraint is needed to reconstruct the 3-D structure from the projections. The conjugate gradient method with the object boundary constraint which is a priori known meets this problem. Experimental results with a biological sample verify the capability of the proposed method as optical tomography of microscopic size.

Citation Download Citation

Satoshi Kawata, Yusuke Touki, and Shigeo Minami "Optical Microscopic Tomography", Proc. SPIE 0558, Inverse Optics II, (25 October 1985); https://doi.org/10.1117/12.949566

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