Combined multiphoton and optical coherence microscopy

Shuo Tang; Tatiana B. Krasieva; Zhongping Chen; Bruce J. Tromberg

doi:10.1117/12.591257

30 March 2005 Combined multiphoton and optical coherence microscopy

Shuo Tang, Tatiana B. Krasieva, Zhongping Chen, Bruce J. Tromberg

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Proceedings Volume 5700, Multiphoton Microscopy in the Biomedical Sciences V; (2005) https://doi.org/10.1117/12.591257
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Multiphoton microscopy (MPM) and optical coherence tomography (OCT) are two important techniques for non-invasive and high-resolution imaging of highly scattering biomedical tissues. MPM and OCT provide complementary information about tissues as the detected signals originate from different substances and mechanisms. Combining MPM and OCT can simultaneously provide rich functionality and morphology information about biomedical tissues. We report a novel system which combines multiphoton and optical coherence microscopy. A femtosecond Ti:Sapphire laser is used as the light source which has a pulse width of 10 fs and a spectral bandwidth of 100 nm. The ultrafast Ti:Sapphire laser provides the short pulse and the broad bandwidth required by high-resolution MPM and OCT, respectively. By matching the resolution and the imaging volume of MPM and OCT, we achieved co-registration between the MPM and OCT images. Dispersion compensation is critical in this MPM/OCT system and its effect is discussed. Representative images of co-registered MPM/OCT are presented.

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Shuo Tang, Tatiana B. Krasieva, Zhongping Chen, and Bruce J. Tromberg "Combined multiphoton and optical coherence microscopy", Proc. SPIE 5700, Multiphoton Microscopy in the Biomedical Sciences V, (30 March 2005); https://doi.org/10.1117/12.591257

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