Application of quantum-dots for analysis of nanosystems by either utilizing or preventing FRET

Joong Hyun Kim; Sumit Chaudhary; Jared P. Stephens; Krishna V. Singh; Mihrimah Ozkan

doi:10.1117/12.602354

28 April 2005 Application of quantum-dots for analysis of nanosystems by either utilizing or preventing FRET

Joong Hyun Kim, Sumit Chaudhary, Jared P. Stephens, Krishna V. Singh, Mihrimah Ozkan

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.602354
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

We have developed conjugates with quantum-dots (QDs) for the purpose of analysis of nanosystems that are organic or inorganic in nature such as DNA and carbon nanotubes. First, by employing Florescence Resonant Energy Transfer (FRET) principles, a hybrid molecular beacon conjugates are synthesized. For water- solubilization of QDs, we modified the surface of CdSe-ZnS core-shell QD by using mercaptoacetic acid ligand. This modification does not affect the size of QDs from that of unmodified QDs. After linking molecular beacons to the carboxyl groups of the modified QDs using 1-Ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride, hybrid molecular beacons are prepared as a DNA probe. After hybridization with specific target DNA and non-specific target DNA, the hybrid conjugates show high specificity to the target DNA with 5-fold increase in the intensity of fluorescence. By developing atomic model of the conjugates, we calculated with 8 numbers of molecular beacons on a single quantum dots, we could increase the efficiency of FRET up to 90%. In other hands, for application of quantum dots to the carbon nanotubes, FRET is a barrier. Thus, after employing 1 % sodium-dodecyl-sulfonate (SDS), single-walled carbon nanotubes are decorated with QDs at their outer surface. This enables fluorescent microscopy imaging of single-walled carbon nanotubes which is a more common technique than electron microscopy. In summary, QDs can be used for analysis or detection of both organic and inorganic based nanosystems.

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Joong Hyun Kim, Sumit Chaudhary, Jared P. Stephens, Krishna V. Singh, and Mihrimah Ozkan "Application of quantum-dots for analysis of nanosystems by either utilizing or preventing FRET", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.602354

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KEYWORDS

Fluorescence resonance energy transfer

Single walled carbon nanotubes

Luminescence

Molecules

Zinc

Quantum dots

Oxygen

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