Functionalized luminescent oxide nanoparticles for sodium channel imaging at the single molecule level

E. Beaurepaire; D. Giaume; V. Buissette; M.-P. Sauviat; D. Casanova; E. Auksorius; J.-L. Martin; K. Lahlil; T. Gacoin; J.-P. Boilot; A. Alexandrou

doi:10.1117/12.590737

28 April 2005 Functionalized luminescent oxide nanoparticles for sodium channel imaging at the single molecule level

E. Beaurepaire, D. Giaume, V. Buissette, M.-P. Sauviat, D. Casanova, E. Auksorius, J.-L. Martin, K. Lahlil, T. Gacoin, J.-P. Boilot, A. Alexandrou

Author Affiliations +

Proceedings Volume 5705, Nanobiophotonics and Biomedical Applications II; (2005) https://doi.org/10.1117/12.590737
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Lanthanide-ion doped oxide nanoparticles were functionalized for use as fluorescent biological labels. These nanoparticles are synthesized directly in water which facilitates their functionalization, and are very photostable without emission intermittency. Nanoparticles functionalized with guanidinium groups act as artificial toxins and specifically target sodium channels. They are individually detectable in cardiac myocytes, revealing a heterogeneous distribution of sodium channels. Functionalized oxide nanoparticles appear as a novel tool particularly well adapted to long-term single-molecule tracking.

Citation Download Citation

E. Beaurepaire, D. Giaume, V. Buissette, M.-P. Sauviat, D. Casanova, E. Auksorius, J.-L. Martin, K. Lahlil, T. Gacoin, J.-P. Boilot, and A. Alexandrou "Functionalized luminescent oxide nanoparticles for sodium channel imaging at the single molecule level", Proc. SPIE 5705, Nanobiophotonics and Biomedical Applications II, (28 April 2005); https://doi.org/10.1117/12.590737

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