In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells

Radostina Alexandrova; Elena Stoykova; Rodica-Mariana Ion; Kristina Nedkova; Elena Ivanova; Kaloian Zdravkov; Georgi Minchev

doi:10.1117/12.618977

22 April 2005 In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells

Radostina Alexandrova, Elena Stoykova, Rodica-Mariana Ion, Kristina Nedkova, Elena Ivanova, Kaloian Zdravkov, Georgi Minchev

Author Affiliations +

Proceedings Volume 5830, 13th International School on Quantum Electronics: Laser Physics and Applications; (2005) https://doi.org/10.1117/12.618977
Event: 13th International School on Quantum Electronics: Laser Physics and Applications, 2004, Bourgas, Bulgaria

Abstract

In this study we evaluate in vitro the cytotoxic activity of two newly synthesized phthalocyanines (metallo-complexes) with absorption bands in the red part of the spectrum: zinc-tri-sulphonated phthalocyanine (ZnS₄Pc). Two permanent animal tumor cell lines are used in the experiments: LSR-SF(SR)-transplantable sarcoma in rat induced by Rous sarcoma virus strain Schmidt-Ruppin and LSCC-SF(Mc29)-transplantable chicken hepatoma induced by the myelocytomatosis virus Mc29. Irradiation has been performed with a laser diode emitting at 672 nm in a wide range of radiant exposure (2-100 J/cm²) and irradiance of 120 mW/cm². The neutral red uptake cytotoxicity assay has been used to evaluate the effect of the photosensitizers on cell viability. The light dose-response curves and light exposures that ensure viability drop to 50% and 10% have been obtained for each cell line. The ability of the compounds to induce apoptosis has been determined by acridine orange dye staining.

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Radostina Alexandrova, Elena Stoykova, Rodica-Mariana Ion, Kristina Nedkova, Elena Ivanova, Kaloian Zdravkov, and Georgi Minchev "In vitro cytotoxicity assessment of phthalocyanines on virus-transformed animal cells", Proc. SPIE 5830, 13th International School on Quantum Electronics: Laser Physics and Applications, (22 April 2005); https://doi.org/10.1117/12.618977

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