Femtosecond laser manipulation of subcellular organelles in living cells

Wataru Watanabe; Tomoko Shimada; Kazuyoshi Itoh; Sachihiro Matsunaga; Kiichi Fukui

doi:10.1117/12.633079

26 August 2005 Femtosecond laser manipulation of subcellular organelles in living cells

Wataru Watanabe, Tomoko Shimada, Kazuyoshi Itoh, Sachihiro Matsunaga, Kiichi Fukui

Proceedings Volume 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II; 58630B (2005) https://doi.org/10.1117/12.633079
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Manipulation—that is, inactivation and movement—of subcellular organelles in living cells is important to understand cell dynamics. We review disruption of subcellular organelles in living cells by tightly focusing femtosecond laser pulses inside the cells. Photodisruption of organelles in living cells was experimentally confirmed by restaining of organelles. Femtosecond laser based nanosurgery has the possibility to remove or manipulate organelles in living cells and may lead to elucidation of functional interactions between organelles, protein transportation, and inter-cell communication.

Citation Download Citation

Wataru Watanabe, Tomoko Shimada, Kazuyoshi Itoh, Sachihiro Matsunaga, and Kiichi Fukui "Femtosecond laser manipulation of subcellular organelles in living cells", Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630B (26 August 2005); https://doi.org/10.1117/12.633079

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