Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers

Ho-Chien Lin; Long Hsu

doi:10.1117/12.616743

26 August 2005 Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers

Ho-Chien Lin, Long Hsu

Proceedings Volume 5930, Optical Trapping and Optical Micromanipulation II; 59301V (2005) https://doi.org/10.1117/12.616743
Event: Optics and Photonics 2005, 2005, San Diego, California, United States

Abstract

Cell sorting is important in quantitative analysis for cellular and molecular biology study. For this purpose, we develop an opto-electro-mechanical sorting system which integrates a holographic optical tweezers (HOT) system to a microfluidics device. This system is designed to manipulate and sort various micro objects of different sizes by an optical pattern which is dynamic and programmable. In this work, we fabricate a microchannel with a main channel and two branches with polydimethylsiloxane (PDMS) which is a biocompatible elastomer material by utilizing semiconductor micro-fabrication techniques. By using a HOT system, we also generate two linear optical traps for the manipulation of micro particles. As a result, we have demonstrated that the larger particles are guided to the second branch by the two linear optical traps while the smaller particles remain unaffected and flow into the first branch of the microchannel.

Citation Download Citation

Ho-Chien Lin and Long Hsu "Dynamic and programmable cell-sorting by using microfluidics and holographic optical tweezers", Proc. SPIE 5930, Optical Trapping and Optical Micromanipulation II, 59301V (26 August 2005); https://doi.org/10.1117/12.616743

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