A study of interaction between FITC- labeled antibody and optical fiber surface

K. F. Chao; Y. L. Zhang; X. G. Kong; Q. H. Zeng; R. L. Liu; X. Wang; L. Y. Feng; Y. J. Sun

doi:10.1117/12.667842

24 January 2006 A study of interaction between FITC- labeled antibody and optical fiber surface

K. F. Chao, Y. L. Zhang, X. G. Kong, Q. H. Zeng, R. L. Liu, X. Wang, L. Y. Feng, Y. J. Sun

Proceedings Volume 6029, ICO20: Materials and Nanostructures; 602922 (2006) https://doi.org/10.1117/12.667842
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

The immobilization of antibodies on solid surface has been widely studied in elucidating immunoassay and in developing immunosensor. In this paper, FITC-labeled goat anti-human IgG were covalently immobilized on 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify the distal end of a fiber-optic probe. The higher density and stability of FITC-labeled goat anti-human IgG could be obtained on the fiber surface modified with APTES-Glu than that on the non-modified fiber surface by evanescent excited fluorescence spectroscopy. Furthermore, red shift of the FITC fluorescence dye emission peak was observed on different fiber surface, exhibiting different interaction between FITC- labeled antibody and surface. Finally, under these conditions antigen-antibody reaction was investigated as an immunosensor. Detection limit of the evanescent wave optic-fiber biosensor is 10ng/ml.

Citation Download Citation

K. F. Chao, Y. L. Zhang, X. G. Kong, Q. H. Zeng, R. L. Liu, X. Wang, L. Y. Feng, and Y. J. Sun "A study of interaction between FITC- labeled antibody and optical fiber surface", Proc. SPIE 6029, ICO20: Materials and Nanostructures, 602922 (24 January 2006); https://doi.org/10.1117/12.667842

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