Effects of a pupil filter on stimulated emission depletion microscopy

Hongki Yoo; Incheon Song; Taehoon Kim; Daegab Gweon

doi:10.1117/12.647395

23 February 2006 Effects of a pupil filter on stimulated emission depletion microscopy

Hongki Yoo, Incheon Song, Taehoon Kim, Daegab Gweon

Proceedings Volume 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII; 60900X (2006) https://doi.org/10.1117/12.647395
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Recently, stimulated emission depletion microscopy has achieved high resolution in fluorescent imaging. In this paper, we present effects of a pupil filter on the performances of stimulated emission depletion microscopy. In stimulated emission depletion microscopy, a saturated zero-centered spot is usually used to achieve a high lateral resolution. Using a half-coated phase plate, a zero-centered spot was made with a narrow and steep gap at the center. Numerical and experimental results show that by simply inserting a central obstacle as a pupil filter, it is possible to reduce the central gap of the zero-centered spot. However in order to compensate inevitable loss of light, which is blocked by the obstacle, increased laser power is required.

Citation Download Citation

Hongki Yoo, Incheon Song, Taehoon Kim, and Daegab Gweon "Effects of a pupil filter on stimulated emission depletion microscopy", Proc. SPIE 6090, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIII, 60900X (23 February 2006); https://doi.org/10.1117/12.647395

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