Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

Valeri I. Kovalev; James S. Barton; Patricia R. Richardson; Anita C. Jones

doi:10.1117/12.646137

27 February 2006 Highly sensitive rapid fluorescence detection of protein residues on surgical instruments

Valeri I. Kovalev, James S. Barton, Patricia R. Richardson, Anita C. Jones

Proceedings Volume 6092, Ultrasensitive and Single-Molecule Detection Technologies; 60920J (2006) https://doi.org/10.1117/12.646137
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

There is a risk of contamination of surgical instruments by nfectious protein residues, in particular, prions which are the agents for Creutzfeldt-Jakob Disease in humans. They are exceptionally resistant to conventional sterilization, therefore it is important to detect their presence as contaminants so that alternative cleaning procedures can be applied. We describe the development of an optimized detection system for fluorescently labelled protein, suitable for in-hospital use. We show that under optimum conditions the technique can detect ~100 zeptomoles/mm² with an area scan speed of ~20 cm²/s and for using the system to detect other agents of biomedical interest. A theoretical analysis and experimental measurements will be discussed.

Citation Download Citation

Valeri I. Kovalev, James S. Barton, Patricia R. Richardson, and Anita C. Jones "Highly sensitive rapid fluorescence detection of protein residues on surgical instruments", Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920J (27 February 2006); https://doi.org/10.1117/12.646137

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