Paper
28 February 2006 Live cell imaging of the endocytosis and the intracellular trafficking of multifunctional lipid nanoparticles
Tieqiao Zhang, S. Narasimhan Danthi, Jianwu Xie, Dehong Hu, Peter Lu, King Li
Author Affiliations +
Abstract
Artificial lipid nanoparticles have drawn great attention due to their potential in medicine. Linked with targeting ligands, they can be used as probes and/or gene delivery vectors for specific types of target cells. Therefore, they are very promising agents in early detection, diagnosis and treatment of cancers and other genetic diseases. However, there are several barriers blocking the applications. Controlling the cellular uptake of the lipid nanoparticles is an important technical challenge to overcome. Understanding the mechanism of the endocytosis and the following intracellular trafficking is very important for improving the design and therefore the efficiency as a drug delivery system. By using fluorescence microscopy methods, we studied the endocytosis of lipid nanoparticles by live M21 cells. The movements of the nanoparticles inside the cell were quantitatively characterized and classified based on the diffusion behavior. The trajectories of nanoparticles movement over the cell membrane revealed hop-diffusion behavior prior to the endocytosis. Fast movement in large steps is observed in intracellular trafficking and is attributed to active movement along microtubule. These observations help to understand the mechanism of the endocytosis and the pathway of the particles in cells.
© (2006) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Tieqiao Zhang, S. Narasimhan Danthi, Jianwu Xie, Dehong Hu, Peter Lu, and King Li "Live cell imaging of the endocytosis and the intracellular trafficking of multifunctional lipid nanoparticles", Proc. SPIE 6095, Nanobiophotonics and Biomedical Applications III, 60950D (28 February 2006); https://doi.org/10.1117/12.645398
Advertisement
Advertisement
RIGHTS & PERMISSIONS
Get copyright permission  Get copyright permission on Copyright Marketplace
KEYWORDS
Nanoparticles

Particles

Luminescence

Microscopy

Live cell imaging

Cancer

Diffusion

Back to Top