Lectin functionalized quantum dots for recognition of mammary tumors

Beate S. Santos; Patricia M. A. de Farias; Frederico D. de Menezes; Ricardo de C. Ferreira; Severino A. Júnior; Regina C. B. Q. Figueiredo D.V.M.; Eduardo I. C. Beltrão

doi:10.1117/12.646819

27 March 2006 Lectin functionalized quantum dots for recognition of mammary tumors

Beate S. Santos, Patricia M. A. de Farias, Frederico D. de Menezes, Ricardo de C. Ferreira, Severino A. Júnior, Regina C. B. Q. Figueiredo D.V.M., Eduardo I. C. Beltrão

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Proceedings Volume 6096, Colloidal Quantum Dots for Biomedical Applications; 60961J (2006) https://doi.org/10.1117/12.646819
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In this study we use CdS/Cd(OH)₂ quantum dots functionalized with concanavalin-A (Con-A) lectin, specific to glucose/mannose residues, to investigate cell alterations regarding carbohydrate profile in human mammary tissues diagnosed as fibroadenoma (benign tumor). These particles were functionalized with glutaraldehyde and Con-A and incubated with tissue sections of normal and to Fibroadenoma, a benign type of mammary tumor. The tissue sections were deparafinized, hydrated in graded alcohol and treated with a solution of Evans Blue in order to avoid autofluorescence. The fluorescence intensity of QD-Con-A stained tissues showed different patterns, which reflect the carbohydrate expression of glucose/mannose in fibroadenoma when compared to the detection of the normal carbohydrate expression. The pattern of unspecific labeling of the tissues with glutaraldehyde functionalized CdS/Cd(OH)₂ quantum dots is compared to the targeting driven by the Con-A lectin. The preliminary findings reported here support the use of CdS/Cd(OH)₂ quantum dots as specific probes of cellular alterations and their use in diagnostics.

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Beate S. Santos, Patricia M. A. de Farias, Frederico D. de Menezes, Ricardo de C. Ferreira, Severino A. Júnior, Regina C. B. Q. Figueiredo D.V.M., and Eduardo I. C. Beltrão "Lectin functionalized quantum dots for recognition of mammary tumors", Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60961J (27 March 2006); https://doi.org/10.1117/12.646819

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