32-channel single photon counting module for ultrasensitive detection of DNA sequences

Georgiy Gudkov; Vinit Dhulla; Anatoly Borodin; Dmitri Gavrilov; Andrey Stepukhovich; Andrey Tsupryk; Boris Gorbovitski; Vera Gorfinkel

doi:10.1117/12.685838

3 November 2006 32-channel single photon counting module for ultrasensitive detection of DNA sequences

Georgiy Gudkov, Vinit Dhulla, Anatoly Borodin, Dmitri Gavrilov, Andrey Stepukhovich, Andrey Tsupryk, Boris Gorbovitski, Vera Gorfinkel

Author Affiliations +

Proceedings Volume 6372, Advanced Photon Counting Techniques; 63720C (2006) https://doi.org/10.1117/12.685838
Event: Optics East 2006, 2006, Boston, Massachusetts, United States

Abstract

We continue our work on the design and implementation of multi-channel single photon detection systems for highly sensitive detection of ultra-weak fluorescence signals, for high-performance, multi-lane DNA sequencing instruments. A fiberized, 32-channel single photon detection (SPD) module based on single photon avalanche diode (SPAD), model C30902S-DTC, from Perkin Elmer Optoelectronics (PKI) has been designed and implemented. Unavailability of high performance, large area SPAD arrays and our desire to design high performance photon counting systems drives us to use individual diodes. Slight modifications in our quenching circuit has doubled the linear range of our system from 1MHz to 2MHz, which is the upper limit for these devices and the maximum saturation count rate has increased to 14 MHz. The detector module comprises of a single board computer PC-104 that enables data visualization, recording, processing, and transfer. Very low dark count (300-1000 counts/s), robust, efficient, simple data collection and processing, ease of connectivity to any other application demanding similar requirements and similar performance results to the best commercially available single photon counting module (SPCM from PKI) are some of the features of this system.

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Georgiy Gudkov, Vinit Dhulla, Anatoly Borodin, Dmitri Gavrilov, Andrey Stepukhovich, Andrey Tsupryk, Boris Gorbovitski, and Vera Gorfinkel "32-channel single photon counting module for ultrasensitive detection of DNA sequences", Proc. SPIE 6372, Advanced Photon Counting Techniques, 63720C (3 November 2006); https://doi.org/10.1117/12.685838

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