Protein profile study of breast cancer tissues using HPLC-LIF: a pilot study

Suraj Menon; Sujatha Raja; K. Kalyan Kumar; Jacob Kurien; B. R. Krishnanand; K. K. Mahato; Sajan D. George; V. B. Kartha; Santhosh Chidangil

doi:10.1117/12.699935

6 February 2007 Protein profile study of breast cancer tissues using HPLC-LIF: a pilot study

Suraj Menon, Sujatha Raja, K. Kalyan Kumar, Jacob Kurien, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, Santhosh Chidangil

Author Affiliations +

Proceedings Volume 6430, Advanced Biomedical and Clinical Diagnostic Systems V; 64300W (2007) https://doi.org/10.1117/12.699935
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Proteomics based techniques are rapidly emerging as alternative techniques to conventional histo-pathological methods for detection and diagnosis of cancers. Tumor markers are of considerable importance in the study of various cancers. A study of various changes in the protein profile associated with breast cancer will facilitate a better understanding of the various dynamic changes associated with the disease. In our study we have used High Performance Liquid Chromatography coupled with highly sensitive Laser Induced Fluorescence for recording the protein profiles of breast tissue homogenates. The protein profiles were recorded from pathologically certified normal as well as malignant breast tissue samples. The recorded protein profiles were studied by using Principal Component Analysis. Good discrimination of normal, benign and malignant samples was achieved in this pilot study.

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Suraj Menon, Sujatha Raja, K. Kalyan Kumar, Jacob Kurien, B. R. Krishnanand, K. K. Mahato, Sajan D. George, V. B. Kartha, and Santhosh Chidangil "Protein profile study of breast cancer tissues using HPLC-LIF: a pilot study", Proc. SPIE 6430, Advanced Biomedical and Clinical Diagnostic Systems V, 64300W (6 February 2007); https://doi.org/10.1117/12.699935

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