Ionic contrast terahertz near-field imaging of axonal activity and water fluxes

Jean-Baptiste Masson; Martin-Pierre Sauviat; Jean-Louis Martin; Guilhem Gallot

doi:10.1117/12.698309

13 February 2007 Ionic contrast terahertz near-field imaging of axonal activity and water fluxes

Jean-Baptiste Masson, Martin-Pierre Sauviat, Jean-Louis Martin, Guilhem Gallot

Proceedings Volume 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V; 644102 (2007) https://doi.org/10.1117/12.698309
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We demonstrated the direct and noninvasive imaging of functional neurons,¹ as well as auricular heart muscle electrical activity² by Ionic Contrast Terahertz (ICT) near-field microscopy. This technique provides quantitative measurements of ionic concentrations in both the intracellular and extracellular compartments and opens the way to direct noninvasive imaging of neurons during electrical, toxin, or thermal stresses. Furthermore, neuronal activity results from both a precise control of transient variations in ionic conductances and a much less studied water exchange between the extracellular matrix and the intraaxonal compartment. The developed ICT technique associated with a full three-dimensional simulation of the axon-aperture near-field system allows a precise measurement of the axon geometry and therefore the direct visualization of neuron swelling induced by temperature change or neurotoxin poisoning. This technique should then provide grounds for the development of advanced functional neuroimaging methods based on diffusion anisotropy of water molecules.

Citation Download Citation

Jean-Baptiste Masson, Martin-Pierre Sauviat, Jean-Louis Martin, and Guilhem Gallot "Ionic contrast terahertz near-field imaging of axonal activity and water fluxes", Proc. SPIE 6441, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, 644102 (13 February 2007); https://doi.org/10.1117/12.698309

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KEYWORDS

Axons

Neurons

Near field

Absorption

Ions

Sodium

Terahertz radiation

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