Two-photon deep imaging through skin and skull of Zebra finches: preliminary studies for in-vivo brain metabolism monitoring

D. Abi-Haidar; T. Olivier; S. Mottin; C. Vignal; N. Mathevon

doi:10.1117/12.701464

12 February 2007 Two-photon deep imaging through skin and skull of Zebra finches: preliminary studies for in-vivo brain metabolism monitoring

D. Abi-Haidar, T. Olivier, S. Mottin, C. Vignal, N. Mathevon

Author Affiliations +

Proceedings Volume 6442, Multiphoton Microscopy in the Biomedical Sciences VII; 64421M (2007) https://doi.org/10.1117/12.701464
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Zebra Finches are songbirds which constitute a model for neuro-ethologists to study the neuro-mechanisms of vocal recognition. For this purpose, in vivo and non invasive monitoring of brain activity is required during acoustical stimulation. MRI (Magnetic Resonance Imaging) or NIRS (Near InfraRed Spectroscopy) are suitable methods for these measurements, even though MRI is difficult to link quantitatively with neural activity and NIRS suffers from a poor resolution. In the particular case of songbirds (whose skin is thin and quite transparent and whose skull structure is hollow), two-photon microscopy enables a quite deep penetration in tissues and could be an alternative. We present here preliminary studies on the feasability of two-photon microscopy in these conditions. To do so, we chose to image hollow fibers, filled with Rhodamine B, through the skin of Zebra finches in order to evaluate the spatial resolution we may expect in future in vivo experiments. Moreover, we used the reflectance-mode confocal configuration to evaluate the exponential decrease of backreflected light in skin and in skull samples. Following this procedure recently proposed by S.L. Jacques and co-workers, we planned to determine the scattering coefficient μ_s and the anisotropy g of these tissues and make a comparison between fixed and fresh skin and skull samples for future Monte Carlo simulations of the scattering in our particular multi-layered structure.

Citation Download Citation

D. Abi-Haidar, T. Olivier, S. Mottin, C. Vignal, and N. Mathevon "Two-photon deep imaging through skin and skull of Zebra finches: preliminary studies for in-vivo brain metabolism monitoring", Proc. SPIE 6442, Multiphoton Microscopy in the Biomedical Sciences VII, 64421M (12 February 2007); https://doi.org/10.1117/12.701464

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
10 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skull

Luminescence

Skin

Reflectivity

Scattering

Confocal microscopy

In vivo imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years