True-color nanosecond time-gated fluorescence imaging using a CCD detector

A. C. Mitchell; E. J. Wall; C. G. Morgan

doi:10.1117/12.710566

14 February 2007 True-color nanosecond time-gated fluorescence imaging using a CCD detector

A. C. Mitchell, E. J. Wall, C. G. Morgan

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430B (2007) https://doi.org/10.1117/12.710566
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We have developed a solid-state true-color charge-coupled (CCD) imager for nanosecond time-gated fluorescence imaging. Unlike intensifier-based imagers, the Peltier-cooled CCD imager is directly gated and hence has very low noise. The excitation source used with the imager is a compact diode-pumped nanosecond pulsed laser giving outputs at 532nm and 355nm with a variable repetition rate up to more than 10kHz. The operation of the new imager has been demonstrated using fluorescent samples that differ both in emission spectrum and in fluorescence decay time. Potential applications of the new technology are discussed including multiplexed detection of labels based on the combination of emission spectrum and fluorescence lifetime.

Citation Download Citation

A. C. Mitchell, E. J. Wall, and C. G. Morgan "True-color nanosecond time-gated fluorescence imaging using a CCD detector", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430B (14 February 2007); https://doi.org/10.1117/12.710566

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