Observation of cell dynamics by laser scanning Raman microscope

Keisaku Hamada; Katsumasa Fujita; Minoru Kobayashi; Satoshi Kawata

doi:10.1117/12.700729

14 February 2007 Observation of cell dynamics by laser scanning Raman microscope

Keisaku Hamada, Katsumasa Fujita, Minoru Kobayashi, Satoshi Kawata

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430Z (2007) https://doi.org/10.1117/12.700729
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We developed a Raman microscope using a slit-scanning technique for observation of biological samples. A sample was illuminated by a line-shaped laser light, and Raman spectra were measured at different points in the line simultaneously by a spectrometer equipped with a 2D detector. The parallel detection of the Raman spectra boosts the image acquisition rate, which enable us to observe a living biological sample with high temporal and spatial resolution. We also applied a noise reduction technique using singular value decomposition. We recorded motion of intracellular components of living HeLa cells as sequential Raman images in a spectral region between 600 - 3000 cm^-1 with the temporal resolution of 3 minutes.

Citation Download Citation

Keisaku Hamada, Katsumasa Fujita, Minoru Kobayashi, and Satoshi Kawata "Observation of cell dynamics by laser scanning Raman microscope", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430Z (14 February 2007); https://doi.org/10.1117/12.700729

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