Autofocus algorithms for digital-holographic microscopy

Patrik Langehanenberg; Björn Kemper; Gert von Bally

doi:10.1117/12.727784

19 July 2007 Autofocus algorithms for digital-holographic microscopy

Patrik Langehanenberg, Björn Kemper, Gert von Bally

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66330E (2007) https://doi.org/10.1117/12.727784
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Digital-holographic metrology enables quantitative phase contrast microscopy of reflective and (partially) transparent samples. In this way, new application fields are opened up for nondestructive investigations of technical samples as well as for marker-free and time-resolved analysis of cell biological processes. Studies on long-term biological processes require permanent focus position readjustment to maintain an optimum image quality. Digital holographic microscopy permits subsequent numerical focusing by variation of the propagation distance. Here, the determination of the optimal propagation distance for a sharply focused image is of particular importance. At the Laboratory of Biophysics image definition quantification algorithms were adapted to the requirements of digital holographic microscopy. In order to obtain robust and reliable algorithms, the object-dependent optical absorption properties were taken into consideration. Automatic focus tracking is demonstrated on investigations with digital holographic microscopy on both technical amplitude objects and cytological pure phase objects.

Citation Download Citation

Patrik Langehanenberg, Björn Kemper, and Gert von Bally "Autofocus algorithms for digital-holographic microscopy", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66330E (19 July 2007); https://doi.org/10.1117/12.727784

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