Fluorescence diffuse optical tomography measurements for tissue engineering

Johanne Desrochers; Patrick Vermette; Réjean Fontaine; Yves Bérubé-Lauzière

doi:10.1117/12.778939

26 October 2007 Fluorescence diffuse optical tomography measurements for tissue engineering

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, Yves Bérubé-Lauzière

Proceedings Volume 6796, Photonics North 2007; 679607 (2007) https://doi.org/10.1117/12.778939
Event: Photonics North 2007, 2007, Ottawa, Canada

Abstract

Currents eorts in tissue engineering (TE) are directed towards growing 3D volumes of tissues. In response to TE needs, we are developing a non-invasive technique based on fluorescence diuse optical tomography (FDOT) to image in 3D, via fluorescence labelling, the formation of micro-blood vessels in tissue cultures grown on biodegradable scaolds in bioreactor conditions. In the present work, we use a non-contact FDOT setup developed for small animal imaging for our measurements. We present experimental results showing the feasability to localize a fluorophore-filled 500μm capillary immersed in a scattering medium contained in a cylindrically-shaped glass tube. These conditions are representative of experiments to be carried on real tissue cultures. Time-resolved scattering-fluorescence measurements are made via Time-Correlated Single Photon Counting (TCSPC) and we use numerical constant fraction discrimination (NCFD) to obtain primary localization information from our time-resolved data.

Citation Download Citation

Johanne Desrochers, Patrick Vermette, Réjean Fontaine, and Yves Bérubé-Lauzière "Fluorescence diffuse optical tomography measurements for tissue engineering", Proc. SPIE 6796, Photonics North 2007, 679607 (26 October 2007); https://doi.org/10.1117/12.778939

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