Highly sensitive evanescent wave combination tapered fiber optic fluorosensor for protein detection

Vincent Nardone; Rakesh Kapoor

doi:10.1117/12.771503

5 February 2008 Highly sensitive evanescent wave combination tapered fiber optic fluorosensor for protein detection

Vincent Nardone, Rakesh Kapoor

Proceedings Volume 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII; 685203 (2008) https://doi.org/10.1117/12.771503
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this paper we are reporting the development of a highly sensitive evanescent wave combination tapered fiber optic fluorosensor. We have demonstrated detection of 5 pM Bovine Serum Albumin (BSA) protein using these fiber optic sensors. The sensor can be easily adopted for detection of other proteins. Six identical probes were prepared and affinity pure Goat anti-BSA antibodies were immobilized on the probe surface. We could detect signal from all the probes kept in 5 pM to 1 nM BSA solution while no signal was detected from the probes kept in 20 nM labeled ESA solution.

Citation Download Citation

Vincent Nardone and Rakesh Kapoor "Highly sensitive evanescent wave combination tapered fiber optic fluorosensor for protein detection", Proc. SPIE 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII, 685203 (5 February 2008); https://doi.org/10.1117/12.771503

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