Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila

Yu-Chia Tsao; Yi-Wen Yang; Woo-Hu Tsai; Tsong-Rong Yan

doi:10.1117/12.758498

7 February 2008 Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila

Yu-Chia Tsao, Yi-Wen Yang, Woo-Hu Tsai, Tsong-Rong Yan

Author Affiliations +

Proceedings Volume 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII; 685206 (2008) https://doi.org/10.1117/12.758498
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Side-polished fiber immunosensor based on surface plasmon resonance (SPR) onto self-assembled protein A layer was proposed for the detection of Legionella pneumophila. A self-assembled protein A layer on gold (Au) surface was fabricated by adsorbing a mixture of 11-mercaptoundecanoic acid (MUA) and activated by N-Ethyl-N'-(3-dimethylaminopropyl) carbodiimide/ N-Hydroxysuccinimide (EDC/NHS). The formation of self-assembled protein A and gold layer on side-polished surface and the binding of antibody and antigen in series were confirmed by SPR response on spectrum. The binding protein A layer can improve the sensitivity, which indirectly supports the configurations that antibody layer is immobilized on the binding protein A layer with a well-ordered orientation. The surface morphology analyses of self-assembled protein A layer on Au substrate and monoclonal antibody against L. pneumophila immobilized on protein A were demonstrated by SPR dip shifts on optical spectrum analyzer. The SPR fiber immunosensor for detection of L. pneumophila was developed and the detection limit was 10 CFU/ml with the SPR dip shift in wavelength from 1070 to 1105nm. The current fabrication technique of a SPR immunosensor using optical fiber for the detection of Legionella pneumophila could be applied to construct other biosensor.

Citation Download Citation

Yu-Chia Tsao, Yi-Wen Yang, Woo-Hu Tsai, and Tsong-Rong Yan "Side-polished fiber immunosensor based on surface plasmon resonance for detection of Legionella pneumophila", Proc. SPIE 6852, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VIII, 685206 (7 February 2008); https://doi.org/10.1117/12.758498

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available