Measurements of elastic constants between probe DNA strands and a target DNA linker

Yun-Hui Park; Sun-Uk Hwang; Daekyung Sung; Dongkyu Kim; Sangyong Jon; Yong-Gu Lee

doi:10.1117/12.794760

29 August 2008 Measurements of elastic constants between probe DNA strands and a target DNA linker

Yun-Hui Park, Sun-Uk Hwang, Daekyung Sung, Dongkyu Kim, Sangyong Jon, Yong-Gu Lee

Proceedings Volume 7038, Optical Trapping and Optical Micromanipulation V; 703804 (2008) https://doi.org/10.1117/12.794760
Event: NanoScience + Engineering, 2008, San Diego, California, United States

Abstract

This paper reports the measurement of elastic constants between two DNA strands that are simultaneously hybridized by a third target-DNA linker. Two probe-DNA strands that are immobilized on fluorescent beads and a target-DNA linker formed a hybridized assembly through the Watson-Crick based pairing. Elastic constants of the resulting assembly were measured using a force calibrated dual optical trap. This study can be used to detect the existence of a target-DNA linker with a specified nucleotide sequence, indicating its potential use in DNA biosensors.

Citation Download Citation

Yun-Hui Park, Sun-Uk Hwang, Daekyung Sung, Dongkyu Kim, Sangyong Jon, and Yong-Gu Lee "Measurements of elastic constants between probe DNA strands and a target DNA linker", Proc. SPIE 7038, Optical Trapping and Optical Micromanipulation V, 703804 (29 August 2008); https://doi.org/10.1117/12.794760

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