In vivo photoacoustic monitoring of photosensitizer in skin: application to dosimetry for antibacterial photodynamic treatment

Akihiro Hirao; Shunichi Sato; Daizoh Saitoh; Nariyoshi Shinomiya; Hiroshi Ashida; Minoru Obara

doi:10.1117/12.808480

12 February 2009 In vivo photoacoustic monitoring of photosensitizer in skin: application to dosimetry for antibacterial photodynamic treatment

Akihiro Hirao, Shunichi Sato, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, Minoru Obara

Author Affiliations +

Proceedings Volume 7177, Photons Plus Ultrasound: Imaging and Sensing 2009; 71772C (2009) https://doi.org/10.1117/12.808480
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

To obtain efficient antibacterial photodynamic effect in traumatic injuries such as burns, depth-resolved dosimetry of photosensitizer is required. In this study, we performed dual-wavelength photoacoustic (PA) measurement for rat burned skins injected with a photosensitizer. As a photosensitizer, methylene blue (MB) or porfimer sodium was injected into the subcutaneous tissue in rats with deep dermal burn. The wound was irradiated with red (665 nm or 630 nm) pulsed light to excite photosensitizers and green (532 nm) pulsed light to excite blood in the tissue; the latter signal was used to eliminate blood-associated component involved in the former signal. Acoustic attenuation was also compensated from the photosensitizer-associated PA signals. These signal processing was effective to obtain high-contrast image of a photosensitizer in the tissue. Behaviors of MB and porfimer sodium in the tissue were compared.

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Akihiro Hirao, Shunichi Sato, Daizoh Saitoh, Nariyoshi Shinomiya, Hiroshi Ashida, and Minoru Obara "In vivo photoacoustic monitoring of photosensitizer in skin: application to dosimetry for antibacterial photodynamic treatment", Proc. SPIE 7177, Photons Plus Ultrasound: Imaging and Sensing 2009, 71772C (12 February 2009); https://doi.org/10.1117/12.808480

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KEYWORDS

Sodium

Skin

Tissue optics

Photoacoustic spectroscopy

Blood

In vivo imaging

Tissues

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