Live TIRF microscopy at 100nm resolution through structured illumination

P. Kner; B. Chhun; E. Griffis; L. Winoto; L. Shao; M. G. L. Gustafsson

doi:10.1117/12.812351

3 March 2009 Live TIRF microscopy at 100nm resolution through structured illumination

P. Kner, B. Chhun, E. Griffis, L. Winoto, L. Shao, M. G. L. Gustafsson

Proceedings Volume 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI; 718417 (2009) https://doi.org/10.1117/12.812351
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Linear Structured Illumination is a powerful technique for increasing the resolution of a fluorescence microscope by a factor of two beyond the diffraction limit. Previously this technique has only been used to image fixed samples because the implementation, using a mechanically rotated fused silica grating, was too slow. Here we describe a microscope design, using a ferroelectric spatial light modulator to structure the illumination light, capable of linear structured illumination at frame rates up to 11Hz. We show live imaging of GFP labeled Tubulin and Kinesin in Drosophila S2 cells.

Citation Download Citation

P. Kner, B. Chhun, E. Griffis, L. Winoto, L. Shao, and M. G. L. Gustafsson "Live TIRF microscopy at 100nm resolution through structured illumination", Proc. SPIE 7184, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI, 718417 (3 March 2009); https://doi.org/10.1117/12.812351

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available