In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents

Kevin G. Phillips; Ravikant Samatham; Philippe Thuillier; Steven L. Jacques

doi:10.1117/12.809674

24 February 2009 In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents

Kevin G. Phillips, Ravikant Samatham, Philippe Thuillier, Steven L. Jacques

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Proceedings Volume 7187, Biomedical Applications of Light Scattering III; 71870S (2009) https://doi.org/10.1117/12.809674
Event: SPIE BiOS, 2009, San Jose, California, United States

Abstract

Using a reflectance mode confocal scanning laser microscope (rCSLM), a noninvasive technique to monitor epidermal thickness in vivo is presented. The modality is characterized by the collection of the reflectance profile from cutaneous tissues, resolved in transverse (x, y) directions at each depth (z) increment. Due to the different light scattering properties of the skin layers, each layer can be identified. The depth of each layer can then be inferred from the axial reflectance profile along the z direction. In pilot experiments an agent that induced epidermal proliferation, 12-O-tetradecanoylphorbol-13-acetate (TPA), was applied topically to the ear of a mouse. Peak-to-valley analysis of the rCSLM A-scans showed the epidermal thickness increasing from an initial 5.4 [μm] to 12.4 [μm] after 24 [hr]. Peak-to-peak analysis showed an increase from 9.1 [μm] to 21.2 [μm]. These results suggest that rCSLM imaging provides a means to study morphologic changes in the epithelium arising from inflammatory response and cell proliferation in vivo without recourse to biopsy or sacrifice of animals.

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Kevin G. Phillips, Ravikant Samatham, Philippe Thuillier, and Steven L. Jacques "In vivo measurements of epidermal thickness by reflectance mode confocal microscopy to assess cellular proliferation induced by topical agents", Proc. SPIE 7187, Biomedical Applications of Light Scattering III, 71870S (24 February 2009); https://doi.org/10.1117/12.809674

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KEYWORDS

Confocal microscopy

Reflectivity

Ear

In vivo imaging

Skin

Cancer

Tissues

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