Label-free DNA methylation analysis using the optofluidic ring resonator sensor

Jonathan D. Suter; Daniel J. Howard; Charles W. Caldwell; Huidong Shi; Xudong Fan

doi:10.1117/12.818562

29 April 2009 Label-free DNA methylation analysis using the optofluidic ring resonator sensor

Jonathan D. Suter, Daniel J. Howard, Charles W. Caldwell, Huidong Shi, Xudong Fan

Proceedings Volume 7322, Photonic Microdevices/Microstructures for Sensing; 732208 (2009) https://doi.org/10.1117/12.818562
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for the purpose of analyzing the degree of methylation in sample oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, is prone to abnormal behavior that may lead to uncontrolled suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool to explore and monitor the degree of methylation in DNA. Two different approaches are explored, using either bisulfite modification or immunoprecipitation. The methods are compared and the signal response for both methods is characterized.

Citation Download Citation

Jonathan D. Suter, Daniel J. Howard, Charles W. Caldwell, Huidong Shi, and Xudong Fan "Label-free DNA methylation analysis using the optofluidic ring resonator sensor", Proc. SPIE 7322, Photonic Microdevices/Microstructures for Sensing, 732208 (29 April 2009); https://doi.org/10.1117/12.818562

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