Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line

A. Petri; M. Kyriazi; E. Alexandratou; M. Rallis; S. Gräfe; D. Yova

doi:10.1117/12.831881

17 July 2009 Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line

A. Petri, M. Kyriazi, E. Alexandratou, M. Rallis, S. Gräfe, D. Yova

Proceedings Volume 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV; 73731I (2009) https://doi.org/10.1117/12.831881
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

In this paper the cytotoxic effect of m-THPC, Foscan®, as well as of the liposomal formulation of m-THPC, Fospeg®,(kind offer of Biolitec) were studied post PDT in the human prostate cancer cell line LNCaP. The cells were incubated for 24h with 0.15 μg/ml and 1.2 μg/ml Foscan® and Fospeg®. Irradiation was performed with a 652nm laser and energy doses 180, 360 and 540mJ/cm². The effect was assessed by the MTT viability test 24h after irradiation. Also the intracellular localization of Foscan® and Fospeg® was monitored by using Laser Scanning Confocal Microscopy Imaging. The results showed no dark toxicity either with Foscan® or Fospeg® at any concentration. Also irradiation at each energy dose in the absence of any photosensitizer, did not affect cellular viability. The cellular death caused after Photodynamic Treatment was dependent on m-THPC concentration and formulation, as well as the delivered energy dose. Fospeg® was more effective as LD50 was achieved with 0.15μg/ml at 180mJ/cm² while for the same cytotoxic result 1.2μg/ml Foscan® was needed. Images from confocal microscopy revealed higher fluorescence intensity in the cytoplasm after incubation with Fospeg®, than upon incubation with Foscan® under the same experimental conditions.

Citation Download Citation

A. Petri, M. Kyriazi, E. Alexandratou, M. Rallis, S. Gräfe, and D. Yova "Evaluation of the PDT effect of Foscan and Fospeg in the LNCaP human prostate cancer cell line", Proc. SPIE 7373, Therapeutic Laser Applications and Laser-Tissue Interactions IV, 73731I (17 July 2009); https://doi.org/10.1117/12.831881

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