Novel type 1 photosensitizers: viability of leukemia cells exposed to reactive intermediates generated in situ by in vitro photofragmentation

Raghavan Rajagopalan; Amol Karwa; Przemyslaw M. Lusiak; Kripa Srivastava; Amruta R. Poreddy; Raghootama S. Pandurangi; Karen P. Galen; William L. Neumann; Gary E. Cantrell; Richard B. Dorshow

doi:10.1117/12.822840

13 July 2009 Novel type 1 photosensitizers: viability of leukemia cells exposed to reactive intermediates generated in situ by in vitro photofragmentation

Raghavan Rajagopalan, Amol Karwa, Przemyslaw M. Lusiak, Kripa Srivastava, Amruta R. Poreddy, Raghootama S. Pandurangi, Karen P. Galen, William L. Neumann, Gary E. Cantrell, Richard B. Dorshow

Author Affiliations +

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 738027 (2009) https://doi.org/10.1117/12.822840
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

Photodynamic therapy of tumors involving Type 2 photosenstizers has been conspicuously successful, but the Type 1 process, in contrast, has not received much attention despite its considerable potential. Accordingly, several classes of molecules containing fragile bonds such as azido (-N=N=N), azo (-N=N-), sulfenato (-S-O-) and oxaza (-N-O-) functional groups that produce reactive intermediates such as radicals and nitrenes upon photoexcitation were prepared and tested for cell viability using U397 leukemia cell line. The azido photosensitizer was conjugated to leukemia cell binding peptide, SFFWRLS, for targeted cell viability study. The cells were incubated with the photosensitizer at various concentrations, and were illuminated for 5, 10, and 20 minutes. The results show that all the photosensitizers caused cell death compared to the controls when exposed to both the photosensitizers and light. Most importantly, selective cell death was observed with the azido peptide conjugate 6, which clearly demonstrates that these Type 1 sensitizers are useful for phototherapeutic applications.

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Raghavan Rajagopalan, Amol Karwa, Przemyslaw M. Lusiak, Kripa Srivastava, Amruta R. Poreddy, Raghootama S. Pandurangi, Karen P. Galen, William L. Neumann, Gary E. Cantrell, and Richard B. Dorshow "Novel type 1 photosensitizers: viability of leukemia cells exposed to reactive intermediates generated in situ by in vitro photofragmentation", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 738027 (13 July 2009); https://doi.org/10.1117/12.822840

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