A sensitive solid-phase time-resolved fluorescence immunoassay apparatus

Yun-lei Wang; Ke-fei Song; Wei-lai Zhang; Jun-ling Li

doi:10.1117/12.835224

28 August 2009 A sensitive solid-phase time-resolved fluorescence immunoassay apparatus

Yun-lei Wang, Ke-fei Song, Wei-lai Zhang, Jun-ling Li

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 73821V (2009) https://doi.org/10.1117/12.835224
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

In the device, a He-Ne laser of flash frequency 1-20 Hz was adopted as exciting light source, and three key technical problems have been solved successfully in order to enhance the detecting sensitivity and measuring stability of the device for time-resolved fluorimmunoassays(TRFIA)^[1]. The first one is to design optimum exciting optical system, so that the exciting light beam excite the sample most effectively. The second one is to have a project spectrum filter which can reduce the affection of the background light to the photomultiplier tube and also ensure influence of the stray light and mixed diffusion light to the sample fluorescence to the least, the sample fluorescence through the integrating sphere and come to the grating monochromator, The right wavelength will be chosed through changing the angle of incidence of the grating monochromator. The third one is to simulate the principle of sample averaging of BOXCAR averager. In the device, SCM was used as primary controller and CPLD was used as timing controller. Through the preparation process, signal-to-noise ratio(SNR) will be improved, also adjust delay time, ampling frequency and sampling number arbitrarily. By testing, the sensitivity is 10-¹²mol/L(substance marked by Eu³⁺), examination repeat is ≤2.5%, examination linearity is from 10^-8mol/L to 10^-12mol/L, correlation coefficient is 99.98%(p≤0.01). The instrument is advanced for ultrasensitive detection of antigen and antibody , and solve the tumor, genetic variation, the virus protein detection.

Citation Download Citation

Yun-lei Wang, Ke-fei Song, Wei-lai Zhang, and Jun-ling Li "A sensitive solid-phase time-resolved fluorescence immunoassay apparatus", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 73821V (28 August 2009); https://doi.org/10.1117/12.835224

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Time resolved spectroscopy

Monochromators

Signal processing

Data centers

Data conversion

Holography

Show All Keywords

Keywords/Phrases

Search In:

Publication Years