Detection of reactive oxygen species in mainstream cigarette smoke by a fluorescent probe

Li Liu; Shi-jie Xu; Song-zhan Li

doi:10.1117/12.835678

28 August 2009 Detection of reactive oxygen species in mainstream cigarette smoke by a fluorescent probe

Li Liu, Shi-jie Xu, Song-zhan Li

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 738236 (2009) https://doi.org/10.1117/12.835678
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

A mass of reactive oxygen species(ROS) are produced in the process of smoking. Superfluous ROS can induce the oxidative stress in organism, which will cause irreversible damage to cells. Fluorescent probe is taken as a marker of oxidative stress in biology and has been applied to ROS detection in the field of biology and chemistry for high sensitivity, high simplicity of data collection and high resolution. As one type of fluorescent probe, dihydrorhodamine 6G (dR6G) will be oxidized to the fluorescent rhodamine 6G, which could be used to detect ROS in mainstream cigarette smoke. We investigated the action mechanism of ROS on dR6G, built up the standard curve of R6G fluorescence intensity with its content, achieved the variation pattern of R6G fluorescence intensity with ROS content in mainstream cigarette smoke and detected the contents of ROS from the 4 types of cigarettes purchased in market. The result shows that the amount of ROS has close relationship with the types of tobacco and cigarette production technology. Compared with other detecting methods such as electronic spin resonance(ESR), chromatography and mass spectrometry, this detection method by the fluorescent probe has higher efficiency and sensitivity and will have wide applications in the ROS detection field.

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Li Liu, Shi-jie Xu, and Song-zhan Li "Detection of reactive oxygen species in mainstream cigarette smoke by a fluorescent probe", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 738236 (28 August 2009); https://doi.org/10.1117/12.835678

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KEYWORDS

Luminescence

Oxygen

Fluorescence spectroscopy

Biology

Chemistry

Infrared spectroscopy

Mass spectrometry

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