Orientation determination of single molecules by highly focused generalized cylindrical vector beams

Xianghui Wang; Shengjiang Chang; Lie Lin; Linrui Wang; Shujuan Hao

doi:10.1117/12.830689

28 August 2009 Orientation determination of single molecules by highly focused generalized cylindrical vector beams

Xianghui Wang, Shengjiang Chang, Lie Lin, Linrui Wang, Shujuan Hao

Proceedings Volume 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging; 738239 (2009) https://doi.org/10.1117/12.830689
Event: International Symposium on Photoelectronic Detection and Imaging 2009, 2009, Beijing, China

Abstract

The orientation of the dipole moment, which is one of important parameters in single-molecule fluorescence spectroscopy, is determined by highly focused generalized cylindrical vector beams combined with a confocal far-field microscope. A generalized cylindrical vector beam can be decomposed into a linear superposition of radially polarized and azimuthally polarized components and the ratio of the radial and longitudinal components of the focal electric field can be adjusted by changing the polarization direction. As compared to the case of a radially polarized incident beam, numerical results demonstrate that fluorescence intensity difference among single molecules with different orientations can be effectively overcome for a particular polarization direction of the generalized cylindrical vector beam, which may increases the signal-to-noise ratio of the practical experiments and then ease the difficulty in orientation determination of single molecules.

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Xianghui Wang, Shengjiang Chang, Lie Lin, Linrui Wang, and Shujuan Hao "Orientation determination of single molecules by highly focused generalized cylindrical vector beams", Proc. SPIE 7382, International Symposium on Photoelectronic Detection and Imaging 2009: Laser Sensing and Imaging, 738239 (28 August 2009); https://doi.org/10.1117/12.830689

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KEYWORDS

Molecules

Luminescence

Polarization

Confocal microscopy

Microscopes

Signal to noise ratio

Superposition

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