Dynamic and label-free high-throughput detection of biomolecular interactions based on phase-shift interferometry

Qiang Li; Guoliang Huang; Wupeng Gan; Shengyi Chen

doi:10.1117/12.845392

28 October 2009 Dynamic and label-free high-throughput detection of biomolecular interactions based on phase-shift interferometry

Qiang Li, Guoliang Huang, Wupeng Gan, Shengyi Chen

Author Affiliations +

Proceedings Volume 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009); 75190H (2009) https://doi.org/10.1117/12.845392
Event: Photonics and Optoelectronics Meetings 2009, 2009, Wuhan, China

Abstract

Biomolecular interactions can be detected by many established technologies such as fluorescence imaging, surface plasmon resonance (SPR)^[1-4], interferometry and radioactive labeling of the analyte. In this study, we have designed and constructed a label-free, real-time sensing platform and its operating imaging instrument that detects interactions using optical phase differences from the accumulation of biological material on solid substrates. This system allows us to monitor biomolecular interactions in real time and quantify concentration changes during micro-mixing processes by measuring the changes of the optical path length (OPD). This simple interferometric technology monitors the optical phase difference resulting from accumulated biomolecular mass. A label-free protein chip that forms a 4×4 probe array was designed and fabricated using a commercial microarray robot spotter on solid substrates. Two positive control probe lines of BSA (Bovine Serum Albumin) and two experimental human IgG and goat IgG was used. The binding of multiple protein targets was performed and continuously detected by using this label-free and real-time sensing platform.

Citation Download Citation

Qiang Li, Guoliang Huang, Wupeng Gan, and Shengyi Chen "Dynamic and label-free high-throughput detection of biomolecular interactions based on phase-shift interferometry", Proc. SPIE 7519, Eighth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2009), 75190H (28 October 2009); https://doi.org/10.1117/12.845392

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available