Multiplex detection of disease marker proteins with arrayed imaging reflectometry

Amrita Yadav; Rashmi Sriram; Benjamin L. Miller

doi:10.1117/12.847363

17 February 2010 Multiplex detection of disease marker proteins with arrayed imaging reflectometry

Amrita Yadav, Rashmi Sriram, Benjamin L. Miller

Proceedings Volume 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems; 75530J (2010) https://doi.org/10.1117/12.847363
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Arrayed Imaging Reflectometry, or "AIR", is a new label-free optical technique for detecting proteins. AIR relies on binding-induced changes in the response of an antireflective coating on the surface of a silicon chip. Thus far, we have demonstrated the use of AIR for the detection of pathogenic E. coli, and for multiplex detection of a broad range of proteins in human serum. Creation of the near-perfect antireflective coating on the surface of silicon requires careful control over preparation of the chip surface prior to probe molecule immobilization. We present methods for highly reproducible, solution-phase silanization and glutaraldehyde functionalization of silicon chips carrying a layer of thermal oxide. Following functionalization with antibodies and passivation of remaining reactive groups, these surfaces provide exceptional performance in the AIR assay.

Citation Download Citation

Amrita Yadav, Rashmi Sriram, and Benjamin L. Miller "Multiplex detection of disease marker proteins with arrayed imaging reflectometry", Proc. SPIE 7553, Frontiers in Pathogen Detection: From Nanosensors to Systems, 75530J (17 February 2010); https://doi.org/10.1117/12.847363

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