Percutaneous fiber-optic sensor for the detection of chemotherapy-induced apoptosis in vivo

James O'Kelly; Kuo-Chih Liao; William Clifton; Daning Lu; Phillip Koeffler; Gerald Loeb

doi:10.1117/12.839834

22 February 2010 Percutaneous fiber-optic sensor for the detection of chemotherapy-induced apoptosis in vivo

James O'Kelly, Kuo-Chih Liao, William Clifton, Daning Lu, Phillip Koeffler, Gerald Loeb

Proceedings Volume 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII; 75551H (2010) https://doi.org/10.1117/12.839834
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Early imaging of tumor response to chemotherapy has the potential for significant clinical benefits. We are developing a family of fiber-optic sensors called Sencils^TM (sensory cilia), which are disposable, minimally invasive, and can provide in vivo monitoring of various analytes for several weeks. The objective of this study was to develop and test our sensor to image the labeling of phosphatidylserine by apoptotic cells in response to chemotherapeutic drugs. FM1-43 was a better fluorescent marker for detecting phosphatidylserine expression than Annexin V-FITC; both the proportion of labeled cells (Annexin V, 15%; FM1-43, 58%) and the relative fluorescent increase (Annexin V-FITC, 1.5-fold; FM1-43, 4.5-fold) was greater when FM1-43 was used to detect apoptosis. Initial testing of the optical sensing technology using Taxol-treated MCF-7 cells demonstrated that injection of FM1-43 resulted in a rapid, transient increase in fluorescence that was greater in apoptotic cells compared to control cells (apoptotic cells, 4-fold increase; control cells, 2-fold increase). Using an established animal model, mice were injected with cyclophosphamide and hepatic apoptosis was assessed by imaging of PS expression. Both the amplitude of fluorescence increase and the time taken for the amplitude to decay to half of its peak were increased in livers from animals treated with cyclophosphamide. Our optical sensing technology can be used to detect the early apoptotic response of cells to chemotherapeutic drugs both in vitro and in vivo. This novel technology represents a unique option for the imaging of tumor responses in vivo, and provides an inexpensive, specific system for the detection of early-stage apoptosis.

Citation Download Citation

James O'Kelly, Kuo-Chih Liao, William Clifton, Daning Lu, Phillip Koeffler, and Gerald Loeb "Percutaneous fiber-optic sensor for the detection of chemotherapy-induced apoptosis in vivo", Proc. SPIE 7555, Advanced Biomedical and Clinical Diagnostic Systems VIII, 75551H (22 February 2010); https://doi.org/10.1117/12.839834

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