Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

Dayong Jin; James Piper; Jingli Yuan; Robert Leif

doi:10.1117/12.841569

24 February 2010 Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

Dayong Jin, James Piper, Jingli Yuan, Robert Leif

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756819 (2010) https://doi.org/10.1117/12.841569
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

Citation Download Citation

Dayong Jin, James Piper, Jingli Yuan, and Robert Leif "Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756819 (24 February 2010); https://doi.org/10.1117/12.841569

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