Applying optical Fourier filtering to standard optical projection tomography

Raoul-Amadeus Lorbeer; Heiko Meyer; Marko Heidrich; Holger Lubatschowski; Alexander Heisterkamp

doi:10.1117/12.841599

24 February 2010 Applying optical Fourier filtering to standard optical projection tomography

Raoul-Amadeus Lorbeer, Heiko Meyer, Marko Heidrich, Holger Lubatschowski, Alexander Heisterkamp

Proceedings Volume 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII; 75700F (2010) https://doi.org/10.1117/12.841599
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

Light microscopy is one of the major tools in modern biology. The steady development of new microscopic techniques leads to an correspondent improvement of biological methods. To expand the catalog of biological experiments, we investigate the possibilities of optical projection tomography (OPT). This technique is based on the already established X-Ray computed tomography. In contrast to most other three-dimensional microscopy techniques it is able to create three dimensional data sets of the specimens natural absorption, staining and fluorescence. Unfortunately, these advantages are opposed by a low resolution, reconstruction artifacts, and a relatively big loss of fluorescence light. We reduced the disadvantage in resolution by applying physical filters in the Fourier plane of the image path, which is not possible in X-Ray imaging yet.

Citation Download Citation

Raoul-Amadeus Lorbeer, Heiko Meyer, Marko Heidrich, Holger Lubatschowski, and Alexander Heisterkamp "Applying optical Fourier filtering to standard optical projection tomography", Proc. SPIE 7570, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVII, 75700F (24 February 2010); https://doi.org/10.1117/12.841599

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